Purification and Characterization of a New Type of a - Glucosidase from Paecilomyces lilacinus That Has Transglucosylation Activity to Produce a - 1 , 3 - and a - 1 , 2 - Linked Oligosaccharides jsem

A fungus producing all a-glucosidase that synthesizes a-1,3and a-1,2-linked glucooligosaccharides by transglucosylation was isolated and identified as Paecilomyces tilacinus. The cell-bound enzyme responsible for the synthesis was extracted by suspension of mycelia with O.1M phosphate buffer (pH 8.0), and the extract was purified. The molecular weight and the isoelectric point were estimated to be 54,OOO and 9.1, respectively. The enzyme was most actiye at pH 5.0 and 650C. The enzyme hydrolyzed maltose, nigerose, and kojibiose. The enzyme also hydrolyzed soluble stErch